Proliferation of human bone marrow stem cells cultured on alfa-tricalcium phosphate cement and titanium discs

Marcel Fasolo de Paris, Carlos Eduardo Baraldi, Christian Viezzer, Denise Cantarelli Machado, Marília Gerhardt de Oliveira

Resumo


Objectives: to evaluate human bone marrow stem cells (HBMSC) cultured on α -tricalcium phosphate cement (α -TCP) and titanium (Ti) discs, as well as the osteoconductive properties of these biomaterials. Methods: discs of α -TCP and commercially pure Ti weremanufactured and placed on polyethylene plates. Cells were cultured according to the following: group I – control (no discs); group II – α-TCP discs; group III – Ti discs. Propidium iodide staining was used to assess cell proliferation. Cells cultures were analyzed at 3,7, 14 and 21 days. Results: Proliferation occurred in both biomaterials. At 3 days, greater cell proliferation was seen in group II than group III (p=0.01). Differences were not significant on the other time periods. Morphologic analysis showed circular morphology in group I, compatible with osteogenic cell lineage. Conclusions: both biomaterials were osteoconductive but not osteoinductive. This did not affect the initial stages of osteogenesis.


Palavras-chave


Stem cells. Calcium phosphates. Titanium.

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DOI: http://dx.doi.org/10.9771/cmbio.v13i1.8992

CAPESCAPES-PERIODICOS CNPq BVS FAPESB UFBA ICS PPORGSISTEM

ISSN(impresso): 1677-5090 / ISSN(eletrônico): 2236-5222